Decorating the surface of Escherichia coli with bacterial lipoproteins: a comparative analysis of different display systems

Abstract Background The display of recombinant proteins on cell surfaces has a plethora applications including vaccine development, screening peptide libraries, whole-cell biocatalysts and biosensor development for diagnostic, industrial or environmental purposes. In the last decades, wide variety surface systems have been developed exposure Escherichia coli , such as autotransporters outer membrane proteins. Results this study, we assess three approaches panel heterologous homologous mature lipoproteins in E. : four from Neisseria meningitidis host strain that are known to be localised inner leaflet membrane. Constructs were made carrying sequences coding eight lipoproteins, each fused delivery portion different systems: autotransporter adhesin involved diffuse adherence-I (AIDA-I) enteropathogenic Lpp’OmpA chimaera truncated form ice nucleation protein (INP), InaK-NC (N-terminal domain with C-terminal one) Pseudomonas syringae. contrast what was observed INP constructs, when AIDA-I Lpp’OmpA, most displayed bacterial both at 37 25 °C demonstrated by FACS analysis, confocal transmission electron microscopy. Conclusions To our knowledge is first study compares using number passenger We shown experimental conditions, choice carrier growth temperature, play an important role translocation onto . Despite all optimization steps performed anchor motif, used not achieved. For proved efficient system although lysis phenotype heterogeneity observed. Finally, found best (especially ones) no inhibition only limited heterogeneity.